Induction and Characterization of UV Resistant Calothrix braunii |
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Authors: | Rashmi Tyagi S. Saxena B. D. Kaushik |
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Affiliation: | 1. National Facility for Blue Groen Algal Collections, Indian Agricultural Research Institute, New Delhi, 110 012, India
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Abstract: | A UV resistant mutant of Calothrix braunii has been isolated after repeated exposure to UV-C (254 nm) radiation. LD50 for wild type against UV-B was 1.74 hand 100% lethality was achieved after 3.5 h. Whereas, UV resistant mutant showed LD50 at 3.33 h and loss of complete survival after 5 h exposure. The growth rate of mutant was about 29 per cent greater than that of the wild type. The photosynthetic pigments, chlorophyll a and phycoerythrin were stimulated by 36.2 and 41.2 per cent, respectively over wild type. There were no differences in nitrogenase activity nitrate reductase activity, extracellular ammonia production and of plasmid DNA. |
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