Trehalose assimilation and turnover inTorulopsis glabrata

Torulopsis glabrata cells have a specific mechanism for endogenous trehalose mobilization that is associated with the lag phase attending new growth. The degree of internal trehalose depletion depends upon the initial concentration of glucose in the medium. Exogenous trehalose was shown to be assimilated via extra-cytoplasmic hydrolysis prior to, transport of the generated glucose into the cytoplasm. The effect on internal trehalose depletion in this case was commensurate with slower presentation of free glucose and was not indicative of the initial concentration of exterrial glucose residues as trehalose. Kinetic parameters for glucose uptake wereK _m =0.49 mM,V _max =109 μmol/min/g dry weight; those for trehalose assimilation wereK _m =2.5 mM,V _max =17.4μmol/min/g dry weight.