A sensitive, continuous spectrophotometric method for assaying alpha-glycerophosphate dehydrogenase: activation by menadione |
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| Authors: | A Garrib W C McMurray |
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| Affiliation: | Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada |
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| Abstract: | ![]()
By use of a more sensitive method than standard assays, it is demonstrated that alpha-glycerophosphate dehydrogenase activity can be monitored continuously in rat liver mitochondria; maximum activity was obtained by sonication in the presence of Triton X-100. Vitamin K3 (menadione) seems to enhance the activity of the enzyme. The assay in the presence of menadione is linear over a much greater mitochondrial concentration (up to 250 micrograms of protein in the reaction mixture). |
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| Keywords: | To whom correspondence and reprint requests should be addressed. |
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