|
|||||
|
|
| 椭圆食粉螨线粒体DNA CO Ⅰ基因片段序列分析 | |
| 引用本文： | 吴太葆,夏斌,邹志文,桂梓,朱志民. 椭圆食粉螨线粒体DNA CO Ⅰ基因片段序列分析[J]. 蛛形学报, 2007, 16(2): 79-82 |
| 作者姓名： | 吴太葆 夏斌 邹志文 桂梓 朱志民 |
| 作者单位： | 南昌大学生命科学学院 南昌330031 |
| 基金项目： | 江西省自然科学基金;江西省教育厅科研项目 |
| 摘 要： | 采用苯酚-氯仿-异戊醇抽提法提取了采自江西南昌和广州潮州2个地理种群的椭圆食粉螨Aleuro-glyphus ovatus Troupeau,1878基因组DNA。以相应引物对椭圆食粉螨线粒体DNA细胞色素氧化酶I亚基基因(mtDNA COI)进行PCR扩增,直接测序,得到379bp的碱基片断(国际基因库索引号EF527825),其碱基序列A、C、G、T含量分别为71bp(18.73%)、53bp(13.98%)、89bp(23.48%)、166bp(43.80%);江西南昌和广州潮州2个地理种群间的椭圆食粉螨mtDNACOI基因片段完全一致,未发现地理差异。 |
| 关 键 词： | 椭圆食粉螨 线粒体DNA CO Ⅰ基因 |
| 修稿时间： | 2007-04-09 |
Analysis of Mitochondrial DNA COI Gene Fragment of Aleuroglyphus ovatus |
|
| WU Tai-Bao,XIA Bin,ZOU Zhi-Wen,GUI Zi,ZHU Zhi-Min. Analysis of Mitochondrial DNA COI Gene Fragment of Aleuroglyphus ovatus[J]. Acta Arachnologica Sinica, 2007, 16(2): 79-82 | |
| Authors: | WU Tai-Bao XIA Bin ZOU Zhi-Wen GUI Zi ZHU Zhi-Min |
| Affiliation: | College of Life Science, Nanchang University, Nanchang 330031,China |
| Abstract: | The genomic DNA of Aleuroglyphus ovatus was extracted by phenol : Chloroform: isoamyl alcohol, The specimens in the present study were collected from NanChang in JiangXi Province and ChaoZhou in GuangZhou Province. In our research , mitochondrial cytochrome oxidase subunit I ( CO I ) gene fragment of Aleuroglyphus ovatus was successfully amplified and sequenced, 379 bp sequences of mtDNA CO I were obtained (accession no. EF527825 in GenBank). The contents of A,C,G and T were 71 bp (18.73 %) ,53 bp (13.98 %) ,89 bp (23.48 %) ,166 bp (43.80 %) , respectively. There is no difference in the CO I gene fragment of Aleuroglyphus ovatus between the two different regions. |
| Keywords: | Aleuroglyphus ovatus mitochondrial DNA CO I gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |