新疆野生油菜胞质雄性不育系1193A对应恢复基因的SSR标记分析

以油菜细胞质雄性不育系1193A和恢复系1193R2为亲本构建F2分离群体,并运用BSA法构建了可育和不育基因池。利用1521对SSR引物进行了多态性分析,结果表明有36对引物在亲本和基因池间都表现多态性,用F2单株验证表明有11对引物与恢复基因连锁,离恢复基因较近的2个标记CB10316和Bn GMS171分布在恢复基因Rf的两侧,遗传距离分别为3.9 c M和5.7 c M,可作为恢复系标记辅助育种的候选标记。

Analysis of restorer gene in male sterile line 1193A with Sinapis arvensis L cytoplasm using SSR marker

In order to study restoring gene, F2 population was builded by male sterile line 1193A with Sinapis arvensis L cytoplasm as female parent and restorer line 1193R2 as male parent. Using SSR marker and bulked segregant analysis (BSA) , polyacrylamide gel electrophoresis were performed to identify genetic linkage markers for 1193A CMS restorer gene and to map the markers. 1521 SSR markers were analyzed and the result showed 36 markers were polymorphic between parents and gene bulks. F2 population detection indicated 8 markers linked to the restore gene, two of which located on both sides of the restore gene and closely linked to the restore gene with a genetic distance of 3.9 cM for CB10316 and 5.7 cM for BnGMS171, respectively, which could be used as candidate markers for marker-assisted breeding of restorer line.