Neutralizing single-chain antibodies against the tick-borne encephalitis virus |
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| Authors: | L N Levanov L E Matveev T E Yun E P Goncharova L R Lebedev A N Shvalov A B Ryzhykov I K Baikov V A Matveeva V A Rikhter N V Tikunova |
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| Institution: | (1) FGUN Vector State Scientific Center of Virology and Biotechnology, pos. Kol’tsovo, Novosibirsk oblast, 630559, Russia;(2) Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia |
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| Abstract: | A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in Escherichia coli cells of the scl3D6 single-chain antibody against the TBE virus. The produced antibodies could bind to the TBE virus, strain 205, and the TBE virus recombinant E protein. The affinity constant of purified scl3D6 was (3.0 ± 0.2) × 107 M?1 for the equilibrium state and (2.8 ± 0.3) × 107 M?1 in the case of antigen-antibody formation on the surface. The obtained single-chain antibody could inhibit the infection potency of the TBE virus on a monolayer of eukaryotic cells. The calculated IC50 value for scl3D6 was 16.7 μg/ml. |
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| Keywords: | affinity single-chain antibodies tick-borne encephalitis virus |
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