Non-invasive methods for determination of cellular growth inPodophyllum hexandrum suspension cultures |
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| Authors: | Saurabh Chattopadhyay V S Bisaria T Scheper A K Srivastava |
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| Institution: | (1) Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, 110016 New Delhi, India;(2) Institut fuer Technische Chemie, Callinstrasse 3, 30167 Hannover, Germany |
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| Abstract: | Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures
ofPodophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear
relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation
in a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass
characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding
is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement
allowed online-in situ biomass monitoring of rather heterogenous plant cell suspension cultures in bioreactor even under the
most desirable pH stat conditions. |
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| Keywords: | Podophyllum hexandrum NADH measurement conductivity measurement on-line biomass measurement |
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