A novel pattern recognition protein of the Chinese oak silkmoth,Antheraea pernyi,is involved in the pro-PO activating system |
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| Authors: | Wang Xialu Zhang Jinghai Chen Ying Ma Youlei Zou Wenjun Ding Guoyuan Li Wei Zhao Mingyi Wu Chunfu Zhang Rong |
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| Institution: | 1.School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning Province 110016.;2.Benxi Institute of Medicines, Shenyang Pharmaceutical University, Benxi, Liaoning Province 117004, PR China |
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| Abstract: | In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein. BMB Reports 2013; 46(7): 358-363] |
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| Keywords: | Antheraea pernyi C-type lectin Innate immunity Pattern recognition protein Pro-PO activation |
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