新城疫病毒F48E8株融合蛋白基因序列分析

本研究报道了新城疫病毒（ＮＤＶ）中国标准强毒株Ｆ４８Ｅ８融合蛋白（Ｆ）基因的序列。该基因核苷酸序列长度为１７００ｂｐ，编码由５５３个氨基酸组成的Ｆ０多肽。Ｆ０酶切激活部位序列为ＲＲＱＲＲ↓Ｆ，具有ＮＤＶ强毒的特征。Ｆ０中有３个主要由疏水性氨基酸组成的区域和６个可糖基化位点。经比较，ＮＤＶＦ４８Ｅ８株和Ｍｉｙａｄｅｒａ株、ＴｅｘａｓＧＢ株的氨基酸同源性分别为９３．６４％和９２．４１％。

SEQUENCE ANALYSIS OF THE FUSION PROTEIN GENE OF NEWCASTLE DISEASE VIRUS STRAIN F 48 E 8

The nucleotide sequence of the gene encoding the fusion (F) protein of the Chinese virulent strain F 48 E 8 of Newcastle disease virus (NDV) was determined. A single open reading frame in the sequence encodes a polypeptide of 553 amino acids which has essential features of the F protein of virulent NDV strains previously established. These include the presence of three magor hydrophobic regions and the structure of Arg-Arg-Gln-Arg-Arg in the cleavage-activation site. A comparison was made among amino acid sequences of the F protein of F 48 E 8 strain and previously published sequences of the Miyadera strain and the Texas GB strain, the homology of 93.64% and 92.41% were observed respectively.