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Thermus sediminis sp. nov., a thiosulfate-oxidizing and arsenate-reducing organism isolated from Little Hot Creek in the Long Valley Caldera,California |
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| Authors: | Zhou En-Min Xian Wen-Dong Mefferd Chrisabelle C Thomas Scott C Adegboruwa Arinola L Williams Nathan Murugapiran Senthil K Dodsworth Jeremy A Ganji Rakesh Li Meng-Meng Ding Yi-Ping Liu Lan Woyke Tanja Li Wen-Jun Hedlund Brian P |
| Institution: | 1.State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People’s Republic of China ;2.School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, 89154, USA ;3.Las Vegas High School PAL Program, Clark County School District, Las Vegas, NV, 89154, USA ;4.Department of Biology, California State University, San Bernardino, San Bernardino, CA, USA ;5.Department of Energy, Joint Genome Institute, Walnut Creek, CA, 94598, USA ;6.Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA ;7.Nevada Institute of Personalized Medicine, University of Nevada, Las Vegas, Las Vegas, NV, 89154, USA ; |
| Abstract: | Thermus species are widespread in natural and artificial thermal environments. Two new yellow-pigmented strains, L198T and L423, isolated from Little Hot Creek, a geothermal spring in eastern California, were identified as novel organisms belonging to the genus Thermus. Cells are Gram-negative, rod-shaped, and non-motile. Growth was observed at temperatures from 45 to 75 °C and at salinities of 0–2.0% added NaCl. Both strains grow heterotrophically or chemolithotrophically by oxidation of thiosulfate to sulfate. L198T and L423 grow by aerobic respiration or anaerobic respiration with arsenate as the terminal electron acceptor. Values for 16S rRNA gene identity (≤?97.01%), digital DNA–DNA hybridization (≤?32.7%), OrthoANI (≤?87.5%), and genome-to-genome distance (0.13) values to all Thermus genomes were less than established criteria for microbial species. The predominant respiratory quinone was menaquinone-8 and the major cellular fatty acids were iso-C15:0, iso-C17:0 and anteiso-C15:0. One unidentified phospholipid (PL1) and one unidentified glycolipid (GL1) dominated the polar lipid pattern. The new strains could be differentiated from related taxa by β-galactosidase and β-glucosidase activity and the presence of hydroxy fatty acids. Based on phylogenetic, genomic, phenotypic, and chemotaxonomic evidence, the novel species Thermus sediminis sp. nov. is proposed, with the type strain L198T (=?CGMCC 1.13590T?=?KCTC XXX). |
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