HIV感染者/AIDS病人高迁移率族蛋白1的表达及其临床意义

通过检测74例处于不同病期的HIV感染者/AIDS患者和10例健康对照者PBMCs中HMGB1 mRNA的表达水平及其外周血浆HMGB1、TNF-a和IL-2水平,比较各组之间表达水平的差异及其与CD4＋T淋巴细胞的关系.发现HMGB1 mRNA的表达水平及血浆HMGB1含量在AIDS病人组明显高于感染者组和正常对照组（P〈0.05）;AIDS患者经HAART治疗后疗效差组HMGB1 mRNA的表达水平及血浆HMGB1含量也明显高于疗效好组（P〈0.05）;而经HAART治疗后效果好且免疫功能恢复的患者HMGB1 mRNA的表达及血浆HMGB1含量均较治疗前明显下降（P〈0.05）;当CD4＋T细胞数低于200/μL时,血浆HMGB1含量以及PBMCs中HMGB1 mRNA表达水平与CD4＋T细胞数呈负相关.显示HMGB1在HIV/AIDS发病及病情进展过程中可能起重要作用,HMGB1血浆含量及PBMCs中HMGB1 mRNA的表达水平高低与HIV/AIDS患者病情轻重密切相关.

The Expression and Clinical Significance of HMGB1 in Patients with HIV Infection/AIDS

To investigate the expression and clinical significance of high mobility group box-1 protein (HMGB1)in patients with HIV infection/AIDS,the expression of HMGB1 mRNA of 74 patients with HIV infection/AIDS of different pathogenic stage and 10 healthy subjects were detected by RT-PCR.And the levels of HMGB 1.TNF-a and IL-2 in plasma were measured by EHSA.The expression of HMGB1 mRNA and levels of plasma HMGB1 in AlDS patients were significantly higher than that in cases with HIV infection or controls respectively(P<0.05).The expression of HMGB1 in AIDS patients with poor efficacy after HAART therapy were significantly higher than that in subjects with good efficacy(P<0.05).And both expression of HMGB1 mRNA and levels of plasma HMGB1 in those AIDS patients,whose immune function rehabilitated to normal level and got good therapy efficacy after HARRT,decreased evidently.Subjects with lower counts of CD4+T cells had hisher expression of HMGB1.and there was negative correlation between CD4+T cell counts and HMGB1 levels when CD4+T cells decreased to less than 200 cells/μL.The result shows that HMGB1 plays probably an important role in the pathogenesis of HIV infection/AIDS and is closely associated to the disease course.