Dehydroepiandrosterone (DHEA) stimulates glucose uptake in rat adipocytes: activation of phospholipase D |
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| Authors: | Ishizawa M Ishizuka T Kajita K Miura A Kanoh Y Kimura M Yasuda K |
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| Institution: | The Third Department of Internal Medicine, Department of General Medicine, Gifu University School of Medicine, Tsukasamachi 40, 500-8705, Gifu, Japan. |
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| Abstract: | We examined the effect of dehydroepiandrosterone (DHEA) on glucose uptake and phospholipase D (PLD) activation in rat adipocytes. DHEA (1 microM) provoked a twofold increase in 3H]2-deoxyglucose (DG) uptake for 30 min. Incorporation of 3H]glycerol into diacylglycerol was increased 150% above basal level for 20 min after stimulation with 1 microM DHEA. DHEA increased PLD activity, measured by the incorporation into 3H]phosphatidylethanol in 3H]palmitate labelled rat adipocytes, or by 3H]choline release in methyl-(3)H]choline labeled rat adipocytes. Our results suggest that DHEA stimulates glucose uptake with activation of PLD in rat adipocytes. |
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