| 萝卜细胞质雄性不育恢复基因的RAPD标记 |
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| 引用本文： | 汪志伟,向长萍,梅时勇,黄来春.萝卜细胞质雄性不育恢复基因的RAPD标记[J].植物遗传资源学报,2004,5(2):153-155. |
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| 作者姓名： | 汪志伟 向长萍 梅时勇 黄来春 |
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| 作者单位： | 1. 华中农业大学园艺林学学院/国家蔬菜改良中心华中分中心,武汉,430070
2. 武汉市蔬菜科学研究所,武汉,430065 |
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| 基金项目： | 湖北省武汉市青年科技晨光计划，湖北省武汉市科技攻关项目 |
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| 摘 要： | 以萝卜恢复系9802和不育系9802A配制杂交组合,并以174株个体组成的F2分离群体作为恢复基因的标记群体.以分离群体的不育株和可育株分别建立不育池和恢复池,利用100个RAPD引物对两池间的多态性进行研究.分析表明引物OPC6在两池间扩增出稳定的多态性差异.经连锁分析,证明标记OPC61900与萝卜细胞质雄性不育恢复基因连锁,遗传距离为11.6cM(Centimorgan).这个标记可应用于对育性恢复基因的标记辅助选择.
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| 关 键 词： | 萝卜 细胞质雄性不育 恢复基因 RAPD标记 |
| 修稿时间： | 2003年10月21 |
RAPD Marker of Restorer Gene for Cytoplasmic Male Sterility in Radish |
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| WANG Zhi wei,XIANG Chang ping,MEI Shi yong,HUANG Lai chun.RAPD Marker of Restorer Gene for Cytoplasmic Male Sterility in Radish[J].Journal of Plant Genetic Resources,2004,5(2):153-155. |
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| Authors: | WANG Zhi wei XIANG Chang ping MEI Shi yong HUANG Lai chun |
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| Institution: | WANG Zhi wei 1,XIANG Chang ping 1,MEI Shi yong 2,HUANG Lai chun 2 |
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| Abstract: | RAPD markers was evaluated in a F 2 population of 174 plants derived from male fertility restorer line 9802 and male sterile line 9802A.Sterile and restorer DNA pools were established using the sterile and fertile plants among F 2 population, respectively. One hundred primers were used for screening polymorphisms between the two pools, and OPC6 produced distinguishable as well as polymorphic band between the two pools. Linkage analysis in the mapping population showed that the marker, OPC6 1900 , was linked to the restorer gene. The distance between the restorer gene and OPC6 1900 was 11.6 cM. The breeding for new fertility restorer lines of CMS radish would be facilitated by using the marker. |
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| Keywords: | Radish Cytoplasmic male sterility Restorer gene RAPD |
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