塔河天然胡杨林地可培养细菌的生态分布研究

目的：研究塔里木河天然胡杨林部分地区可培养细菌的生态分布。方法：通过塔里木河胡杨林采样,可培养菌分离及16S rDNA序列鉴定。结果：从3种不同样品（水样、土样和胡杨树杆分泌物）中分离筛选了22株细菌,其中17株菌为革兰氏阳性菌,5株为革兰氏阴性菌。根据生理生化特征与16S rDNA序列分析结果表明,其中15株菌属于芽孢杆菌属,4株属于不动杆菌属、假单胞菌属、动性球菌属和Agrococcus属各含有1个分离株。结论：塔里木河胡杨林可培养微生物中芽孢杆菌比较丰富,其中有3个可能的新种。

Cultivable Bacterial Diversity in A Small Area of Natural Populus Euphractica Forest of Tarim River Valley, China

Objective: Investigate the bacteria community diversity in a small area of Natrural Populus euphractica Forest of Tarim River Valley, China. Method: Using phenotypic and biochemical characterization, and finally molecular identification by analysis of polymerase chain reaction(PCR)-amphfied 16S rRNA gene sequence.Result: 22 cultivable different bacteria had isolated from soil, waste water and secretion liquid the Poptdus euphractica stand by culturing with LB medium, respectively. Among them 17 strains were gram-positive, 5 strains were gram-negative. Based on analysis of 16S rRNA gene sequence showed that 15 strains found to be Bacilli, 4 strains were Acinetobacter, other than Ba-cilli and Acinetobacter there is one Pseudomonas strain(W1-1), one Planococcus strain (W2-3), and one Agrococcus strain (M5). Conclu-sion:There is a high diversity of Bacilli species in this small area, while some potential novel species were found belong to the Bacillus genus and ornithinibacillus.