IMPROVED Candida methylica FORMATE DEHYDROGENASE FERMENTATION THROUGH STATISTICAL OPTIMIZATION OF LOW-COST CULTURE MEDIA |
| |
| Authors: | Mustafa Kahraman Emel B?çakç? Ordu Koray Ye?iladal? Candan Tamerler |
| |
| Institution: | 1. Faculty of Science and Letters, Department of Molecular Biology and Genetics , Istanbul Technical University , Istanbul , Turkey;2. Istanbul Technical University Molecular Biology-Biotechnology &3. Genetics Research Center (MOBGAM) , Istanbul , Turkey;4. Genetics Research Center (MOBGAM) , Istanbul , Turkey;5. Faculty of Science and Letters, Department of Molecular Biology and Genetics , Y?ld?z Technical University , Istanbul , Turkey |
| |
| Abstract: | NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is of use in the regeneration of NAD(P)H coenzymes, and therefore has strong potential for practical application in chemical and medical industries. A low-cost production of recombinant Escherichia coli (E. coli) containing FDH from Candida methylica (cmFDH) was optimized in molasses-based medium by using response surface methodology (RSM) based on central composite design (CCD). The beet molasses as a sole carbon source, (NH4)2HPO4 as a nitrogen and phosphorus source, KH2PO4 as a buffer agent, and Mg2SO4 · 7H2O as a magnesium and sulfur source were used as variables in the medium. The optimum medium composition was found to be 34.694 g L?1 of reducing sugar (equivalent to molasses solution), 8.536 g L?1 of (NH4)2HPO4, 3.073 g L?1 of KH2PO4, and 1.707 g L?1 of Mg2SO4 · 7H2O. Molasses-based culture medium increased the yield of cmFDH about three times compared to LB medium. The currently developed media has the potential to be used in industrial bioprocesses with low-cost production. |
| |
| Keywords: | biocatalyst preparation Candida methylica culture media formate dehydrogenase statistical optimization |
|
|
