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Fast Purification of Phaseolus Vulgaris Isolectins |
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| Authors: | E Zenteno M Ortega Z Qin J Montreuil H Debray |
| Institution: | 1. Departamento de Bioquímica, Facultad de Medicina , Universidad Nacional Autónoma de México , 04510, México;2. Laboratorio de Biología Experimental , Universidad Autónoma del Estado de Morelos , Cuernavaca, 62170, México;3. Department of Biological Chemistry , Hershey Medical Center, Penn State University , Hershey, PA, 17033, U.S.A.;4. Laboratoire de Chimie Biologique , Unité Mixte de Recherche du CNRS No. 111, Université des Sciences et Technologies de Lille , 59655, Villeneuve d'Ascq, CEDEX, France |
| Abstract: | Abstract Phytohemagglutinin from red kidney bean has been purified by affinity chromatography on a human α1-acid glycoprotein Sepha-rose 4B column. Further purification of the hemagglutinin's five isolectins was achieved on a Mono S column with an 86% protein recovery. Each sequentially eluted isolectin from the ion exchange column displayed either hemagglutinating or mitogenic activity. The main activity of each fraction was the result of the combination of varying proportions of the L and E subunits. |
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