Variations of Some Enzyne and Coenzyme Concentrations in Nornal Human Erythrocytes Fractionated by Velocity Sedimentation |
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| Authors: | Anna Fang Wu Tai Te Wu |
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| Affiliation: | 1. Department of Medicine , Northwestern University Medical School , Chicago, IL, 60611;2. Department of Biochemistry &3. Ffolecular Biology , Northwestern University , Evanston, IL, 60201;4. Department of Engineering Sciences &5. Applied Mathematics , Northwestern University , Evanston, IL, 60201 |
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| Abstract: | Abstract The concentrations of glucose-6-phosphate dehydrogenase, adenosine triphosphate and 2,3-diphosphoglycerate were measured in various fractions of normal human erythrocytes separated by velocity sedimentation in isotonic saline and sucrose buffer based on their tendency to aggregate. The levels of 2,3-diphosphoglycerate varied most drastically in fresh erythrocytes, being lowest in fast sedimenting cells and highest in slowly sedimenting cells. This result implies that the 2,3-diphosphoglycerate concentrations is not constant in all normal human erythrocytes and that 2,3-diphosphoglycerate may be involved in the mechanism of red cell clumping in vitro. |
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