No single way to explain cytoplasmic maturation of oocytes from prepubertal and cyclic gilts |
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| Institution: | 1. Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland;2. Department of Animal Nutrition and Feed Management, Poznan University of Life Sciences, Poznan, Poland;3. Department of Statistical and Mathematical Methods, Poznan University of Medical Science, Poznan, Poland;1. Biotechnology Nucleus of Sobral, Federal University of Ceara, Sobral, CE, Brazil;2. Laboratory of Biotechnology and Animal Reproduction, Federal University of Santa Maria, Santa Maria, RS, Brazil;1. Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz 5166616471, Iran;2. Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, Bonn 53111, Germany;3. Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Braunschweig 38116, Germany;4. Clinic for Cattle, University of Veterinary Medicine, Hannover 30173, Germany;5. Institute of Nutritional Physiology “Oskar Kellner”, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, Dummerstorf 18196, Germany;1. KEK Theory Center, High Energy Accelerator Research Organization (KEK), Japan;2. Graduate University for Advanced Studies (SOKENDAI), Tsukuba, Ibaraki 305-0801, Japan;3. Department of Physics, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan |
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| Abstract: | The objective of this study was to evaluate selected aspects of cytoplasmic maturation in oocytes from prepubertal and cyclic crossbred gilts before and after in vitro maturation. For this purpose, cortical granule redistribution, mitochondrial DNA content and mitochondria translocation were analyzed. Moreover, for the first time the fatty acid profiles in follicular fluid (FF) of both gilt categories was evaluated. The nuclear maturation (the percentage of metaphase II oocytes was 83% in prepubertal gilts compared with 87% in cyclic gilts), cortical granule relocation from the cortex to peripheral ooplasm (98.7% vs. 98.8% of oocytes, respectively) and mitochondrial DNA content (227 543 vs. 206 660, respectively) was not affected by sexual maturity of the donor gilt. However, the redistribution of active mitochondria during in vitro maturation was observed only in the oocytes of cyclic gilts. With regard to FF analysis, saturated, unsaturated, and monounsaturated fatty acids were significantly more abundant in the FF of prepubertal females. In particular, stearic (C18:0) and palmitic (C16:0) fatty acids had significantly higher concentrations in the FF of prepubertal gilts. In conclusion, although the oocytes of prepubertal gilts matured in vitro at a rate similar to those of cyclic gilts, they differed with respect to the selected factors attributed to cytoplasmic maturation. We suggest that the higher content of particular fatty acids, which is known to have a negative influence on oocyte maturation, as well as impaired mitochondria redistribution are factors limiting the maturation potential of oocytes from prepubertal gilts. |
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