Global functional analyses of rice promoters by genomics approaches |
| |
Authors: | Su-May Yu Swee-Suak Ko Chwan-Yang Hong Hsou-Jan Sun Yue-Ie Hsing Chii-Gong Tong Tuan-Hua David Ho |
| |
Institution: | (1) Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, 115, Taiwan, ROC;(2) Academia Sinica Biotechnology Experimental Center in Southern Taiwan, Southern Taiwan Science Park, Tainan, 741, Taiwan, ROC;(3) Department of Agricultural Biochemistry, National Taiwan University, Taipei, 106, Taiwan, ROC;(4) Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, 115, Taiwan, ROC |
| |
Abstract: | Promoters play key roles in conferring temporal, spatial, chemical, developmental, or environmental regulation of gene expression.
Promoters that are subject to specific regulations are useful for manipulating foreign gene expression in plant cells, tissues,
or organs with desirable patterns and under controlled conditions, and have been important for both basic research and applications
in agriculture biotechnology. Recent advances in genomics technologies have greatly facilitated identification and study of
promoters in a genome scale with high efficiency. Previously we have generated a large T-DNA tagged rice mutant library (TRIM),
in which the T-DNA was designed with a gene/promoter trap system, by placing a promoter-less GUS gene next to the right border of T-DNA. GUS activity screens of this library offer in situ and in planta identifications
and analyses of promoter activities in their native configurations in the rice genome. In the present study, we systematically
performed GUS activity screens of the rice mutant library for genes/promoters constitutively, differentially, or specifically
active in vegetative and reproductive tissues. More than 8,200 lines have been screened, and 11% and 22% of them displayed
GUS staining in vegetative tissues and in flowers, respectively. Among the vegetative tissue active promoters, the ratio of
leaf active versus root active is about 1.6. Interestingly, all the flower active promoters are anther active, but with varied
activities in different flower tissues. To identify tissue specific ABA/stress up-regulated promoters, we compared microarray
data of ABA/stress induced genes with those of tissue-specific expression determined by promoter trap GUS staining. Following
this approach, we showed that the peroxidase 1 gene promoter was ABA up-regulated by 4 fold within 1 day of exposure to ABA and its expression is lateral root specific.
We suggest that this be an easy bioinformatics approach in identifying tissue/cell type specific promoters that are up-regulated
by hormones or other factors.
Su-May Yu and Swee-Suak Ko equally contributed to this work. |
| |
Keywords: | Rice Functional genomics Promoter trap ABA Tissue-specific promoters Microarray Peroxidase |
本文献已被 PubMed SpringerLink 等数据库收录! |
|