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Positions of QAand ChlZRelative to Tyrosine YZand YDin Photosystem II Studied by Pulsed EPR
Authors:A Kawamori  N Katsuta  H Mino  A Ishii  J Minagawa  T-A Ono
Institution:(1) Faculty of Science, Kwansei Gakuin University, Gakuen 2-1, Sanda, 669-1337, Japan;(2) Department of Physics, Graduate School of Science, Nagoya University, Chikusa, Nagoya, 464-860, Japan;(3) Laboratory for Photo-Biology, RIKEN Photo-dynamics Research Center, The Institute of Physical and Chemical Research, 519-1399, Japan
Abstract:PELDOR (Pulsed Electron eLectron DOuble Resonance) was applied to determinethe distance of between YZand QA -inYD-less mutant of Chlamydomonas reinhardtiiin Tris-treatedand Zn-substituted preparation of photosystem II. The value of distance wasfound to be 34.5 ± 1 Â. A lsquo2+1rsquo electron spin echo method has beenapplied to measure the orientation of the radius-vector RfomYDto ChlZin a membrane-oriented photosystem II. The anglebetween Rand the membrane normal nwas determined to be 50 ±5°, using the distance 29.4 ± 0.5 Â determined in non-orientedPS II.
Keywords:Chlamydomonas reinhardtii  Chlorophyll Z  positions  pulsed EPR  PS II  QA  YD  YD-less mutant  YZ
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