Highly efficient <Emphasis Type="Italic">in vitro</Emphasis> adventitious shoot regeneration of peppermint (<Emphasis Type="Italic">Mentha</Emphasis> x <Emphasis Type="Italic">piperita</Emphasis> L.) using internodal explants |
| |
| Authors: | Xiaohuan Wang Zhenhua Gao Yunzhen Wang Ray A Bressan Stephen C Weller Xia Li |
| |
| Institution: | (1) The State Key Laboratory of Plant Cell and Chromosome Engineering, Center of Agricultural Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei, 050021, China;(2) Department of Horticulture and Landscape Architecture, Purdue University, 1165 Horticulture Building, West Lafayette, IN 47907, USA;(3) Center of Agricultural Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei, 050021, China; |
| |
| Abstract: | An in vitro regeneration system with a 100% efficiency rate was developed in peppermint Mentha x piperita] using 5- to 7-mm-long second internode stem segments of 3-wk-old stock plants. Shoots developed at sites of excision on
stem fragments either directly from the cells or via primary calluses. The optimal medium for maximum shoot initiation and
regeneration contained Murashige and Skoog (MS) salts, B5 vitamins, thidiazuron (TDZ, 11.35 μM), ZT (4.54 μM), 10% coconut
water (CW), 20 g l−1 sucrose, 0.75% agar, adjusted to pH 5.8. A frequency of 100% shoot initiation was achieved, with an average of 39 shoots
per explant. This regeneration system is highly reproducible. The regenerated plants developed normally and were phenotypically
similar to Black Mitcham parents. |
| |
| Keywords: | Peppermint Mentha x piperita Internodes Organogenesis In vitro regeneration |
| 本文献已被 SpringerLink 等数据库收录！ |
|
