Shoot regeneration from petioles of coral bells (Heuchera sanguinea Engelm.) cultured in vitro, and subsequent planting and flowering ex vitro |
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Authors: | Takashi Hosoki Emiko Kajino |
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Institution: | (1) Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu-cho, 690-8504 Matsue, Shimane Pref., Japan |
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Abstract: | Summary Successful shoot regeneration from petioles, leaves, and petioles with leaves cultured in vitro is reported in Heuchera sanguinea. Petioles or petioles with leaves regenerated more shoots than leaves alone. For culture, the optimum hormonal concentrations
were 0.19 μM α-naphthaleneacetic acid combined with 0.44 or 4.4 μM benzyladenine in Murashige and Skoog-based (MS) medium: the regenerating rate and the number of shoots per explant were 60%
and 8.6–9.7, respectively. Histological study on petiole culture showed dividing cell clusters including vascular tissues
after 1 wk, callus including several dividing cell clusters at the periphery after 3 wk and then apical meristems with immature
leaves after 5 wk. Rooting from the regenerated shoots was highest (95%) on MS medium containing 4.9 μM indole-3-butyric acid. Seventy-three percent of rooted plants were successfully acclimatized in pots. When they were cultured
in the field, the plants grew and most flowered the following year over winter. |
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Keywords: | Heuchera sanguinea regeneration histology tissue culture |
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