Examination of lymphokines induced in mice following immunization with recombinant simian virus 40 large tumor antigen |
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| Authors: | Robert K Bright Michael H Shearer Ronald C Kennedy |
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| Institution: | (1) Department of Virology and Immunology, Southwest Foundation for Biomedical Research, 78228 San Antonio, Texas, USA;(2) Department of Microbiology, The University of Texas Health Science Center, 78284 San Antonio, Texas, USA;(3) Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, College of Medicine, 73190 Oklahoma City, Oklahoma, USA |
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| Abstract: | Baculovirus-derived recombinant simian virus 40 (SV40) large tumor antigen (T-Ag) was used to immunize BALB/c mice to examine the lymphokines produced following immunization. Specifically, we examined production of interleukin-2 (IL-2), IL-4, IL-5 and interferon  (IFN) from immune lymphocytes cultured with decreasing concentrations of recombinant SV40 T-Ag. We identified elevated levels of IFN and IL-2 by enzyme-linked immunosorbent assay and a murine CTLL-2 proliferation biossay respectively. We were unable to detect either IL-4 or IL-5. These data indicate the previously reported tumor immunity induced by recombinant SV40 T-Ag immunization most likely reflects a TH1-like immune response based on thein vitro production of both IFN and IL-2 by immune lymphocytes. |
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| Keywords: | Tumor antigens Tumor immunity Helper T-lymphocytes Interleukin Interferon |
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