肉毒毒素中和抗体的研究进展

肉毒毒素是目前已知毒性最强的细菌蛋白质,极少量便可以致人死亡,我国每年都有散发病例出现,并且它极有可能被用于恐怖行动或被一些国家用作生物战剂。肉毒毒素中和抗体是肉毒毒素中毒后惟一有效的药物。与马源的抗毒素血清相比,重组基因工程中和抗体具有很多优势,是目前肉毒毒素预防和治疗研究的主要方向。简要综述了肉毒毒素基因工程中和抗体研究现状、保护性抗原选择、体内外中和活性检测方法及研发难点、解决方法等。

Perspective in Neutralizing Antibodies Against Botulinum Neurotoxin

Botulinum neurotoxin is the most poisonous protein substance known and a little of toxin can be fatal.The onset of the disease appeared every year in China and it is likely to be used for bioterrorism or biowarfare by some countries.Toxin neutralizing antibody is the unique useful drug for the treatment of postexposure.Compared to antitoxin from immunized horses,genetic engineered neutralizing antibody has many advantages and has been thought as the primary development object for the prevention and therapy of botulinum toxin intoxication.This article described the perspective in neutralizing antibodies against botulinum neutrotoxin including the selection of protective antigens,the methods for detecting the activity of neutralizing antibodies,the difficulties during the development and the resolvent.