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Differential expression of two related organ-specific genes in pea
Authors:Mary E. Williams  John Mundy  Steven A. Kay  Nam-Hai Chua
Affiliation:(1) Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, 10021 New York, NY, USA;(2) Present address: Department of Biotechnology, Carlsberg Research Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark
Abstract:We have screened a pea genomic library using a cDNA probe derived from pea shoot RNA. From this screen, we isolated two closely related genes, designated as S2 and P4. An intriguing property of these two genes is the presence in their coding region of a repeated sequence that is conserved between them in sequence but not in the number of the repeating units. The predicted amino acid sequence suggests that these proteins could be exported and glycosylated. 3prime S1 analysis reveals that one of the genes, S2, is expressed highly in stem, as expected from previous work. However, mRNA derived from the other gene, P4, is not detectable in stem tissue, but is present in tissue derived from pea pods. The 5prime upstream sequence of S2 and P4 are 94% identical up to position -121, suggesting that sequences upstream of -121 are responsible for organ-specific expression of the two genes.
Keywords:pea  tissue-specific gene expression  stem  pod
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