Macaques co-immunized with SIVgag/pol-HIVenv and IL-12 plasmid have increased cellular responses |
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Authors: | Robinson T M Sidhu M K Pavlakis G N Felber B K Silvera P Lewis M G Eldridge J Weiner D B Boyer J D |
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Institution: | Jefferson University, Philadelphia, PA, USA. |
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Abstract: | BACKGROUND: The cell mediated immune profiles following immunization with a recombinant DNA vaccine was assessed in the simian-human immunodeficiency virus (SHIV) and Macaque model. Earlier work demonstrated increased numbers of antigen specific CD8 and CD4 effector cells able to secrete IFN-gamma. METHOD: The vaccine strategy included co-immunization of a DNA based vaccine alone or in combination with a macaque IL-12 expressing plasmid (pmacIL12). Antigen activated lymphocytes were studied for activation of a set of immunological molecules. RESULTS: The current study demonstrates lymphocytes isolated and activated from the group that was immunized with DNA and pmacIL12 had a higher level of IFN-gamma producing cells. We also observed a different immunological profile when comparing the cells isolated from macaques immunized with DNA as compared to those animals that also received pmacIL12. CONCLUSION: The observed immune profiles are reflective of the co-delivery of pmacIL12 and demonstrates that IL-12 can increase the magnitude and polyfunctionality of the cellular immune response. |
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Keywords: | DNA Vaccines HIV-1 IL-12 Macaque SHIV |
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