Developmental defects and seedling lethality in apyrase <Emphasis Type="Italic">AtAPY1</Emphasis> and <Emphasis Type="Italic">AtAPY2</Emphasis> double knockout mutants |
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Authors: | Carolin Wolf Maria Hennig Dwight Romanovicz Iris Steinebrunner |
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Institution: | (1) Department of Biology, Section of Molecular Biotechnology, Technical University of Dresden, 01062 Dresden, Germany;(2) Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA;(3) Present address: Department of Pharmacy, Section of Molecular Cell Biology, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany |
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Abstract: | Previously it was shown that the Arabidopsis apyrase genes AtAPY1 and AtAPY2 are crucial for male fertility because mutant pollen (apy1-1; apy2-1) with T-DNA insertions in both genes could not germinate (Steinebrunner et al. (2003) Plant Physiol. 131: 1638–1647). In this study, pollen germination was restored and apyrase T-DNA double knockouts (DKO)
apy1-1/apy1-1; apy2-1/apy2-1 were generated by complementation with AtAPY2 under the control of a pollen-specific promoter. The DKO phenotype displayed developmental defects including the lack of
functional root and shoot meristems. In cotyledons, morphogenetic and patterning abnormalities were apparent, e.g., unlobed
pavement cells and stomatal clusters. Another set of lines was created which carried either AtAPY1 or AtAPY2 under a dexamethasone-(DEX)-inducible promoter as an additional transgene to the pollen-specific gene construct. Application
of DEX did not reverse the DKO phenotype to wild-type, but some inducible lines exhibited less severe defects even in the
absence of the inducer, probably due to some background expression. However, even these DKO mutants were seedling-lethal and
shared other defects regarding cell division, cell expansion and stomatal patterning. Taken together, the defects in the DKO
mutants demonstrate that AtAPY1 and AtAPY2 are essential for normal plant development. |
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Keywords: | Apyrase Cell division Cell expansion Stomatal patterning Pollen-specific promoter Seedling lethality |
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