Kinetic studies of pyruvate kinase duringin vitro differentiation of GM-CFC haemopoietic precursor and bone marrow cells in mice |
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Authors: | Elvira Cuenllas Soledad Gaitan Juan A Bueren Concepcion Tejero |
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Institution: | (1) Departmento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain;(2) Laboratorio de Biología Molecular y Celular, Centro de Investigaciones Energéticas Medioambientales y Tecnológicas CIEMAT, 28040 Madrid, Spain |
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Abstract: | Pyruvate kinase studies in the granulocyte-macrophage lineage duringin vitro differentiation have been performed using culture techniques on GM-CFC cells and a study has also been done in bone marrow cells.The enzyme exhibits biphasic behaviour with respect to both of its substrates in cells derived fromin vitro cultures at 5 and 7 days of incubation period. However in bone marrow cells these kinetics are only observed for ADP.The different kinetic behaviour of pyruvate kinase toward Fru-1,6-P2, Ala, Phe and ATP in the three cellular populations allows us to conclude that the expression of pyruvate kinase is associated with the differentiation of these cells.Abbreviations GM-CFC
granulocyte-macrophage colony forming cells
- PK
pyruvate kinase
- CFU-E
Colony Forming Units Erythroid
- Ew
Error weight
- PEP
phosphoenolpyruvate
- Fru-1,6-P2
fructose 1,6-bisphosphate
- Ala
L-alanine
- Phe
L-phenylanine
- 5 GM
granulocytemacrophage colonies obtained after 5 days incubation
- 7 GM
granulocyte-macrophage colonies obtained after 7 days incubation
- h
Hill coefficient
- S0,5
substrate concentration that yields half-maximal velocity |
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Keywords: | in vitro GM-CFC culture pyruvate kinase differentiation |
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