Mitochondrial DNA data unveil highly divergent populations within the genus <Emphasis Type="Italic">Hynobius</Emphasis> (Caudata: Hynobiidae) in South Korea |
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Authors: | Hae-Jun Baek Mu-Yeong Lee Hang Lee Mi-Sook Min |
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Institution: | 1.Conservation Genome Resource Bank for Korean Wildlife (CGRB) and Research Institute for Veterinary Science College of Veterinary Medicine,Seoul National University,Seoul,Korea |
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Abstract: | Korean salamanders of the genus Hynobius are currently classified into 3 species, H. leechii, H. quelpaertensis, and H. yangi. To investigate the phylogenetic relationship of these species, we analyzed the partial sequence of mitochondrial cytochrome
b gene (907 bp) of 197 specimens from 43 regions in South Korea. Of these specimens, 93 were additionally examined with 12S
rRNA (799 bp). Based on the partial sequence of the mitochondrial cytochrome b gene and 12S rRNA, 89 and 36 haplotypes were
defined, respectively, consisting of six subclades (H. leechii, H. quelpaertensis, H. yangi, HC1, HC2, and HC3). Among these subclades, the three subclades (HC1, HC2, and HC3) were clearly separated from the 3 previously
reported species in the genus Hynobius. Pairwise sequence divergence between the six subclades ranged from 6.3 to 11.2% in cytochrome b gene and 2.0 to 4.3% in 12S rRNA. These results indicate there may be more divergent populations than the three currently
described. Moreover, the estimation of divergence time revealed that the Hynobius species in South Korea diverged during the
Miocene epoch, approximately 9 — 5 MYA. In addition, we confirmed the distribution of the three known species (H. leechii, H. quelpaertensis, and H. yangi) and determined the distributions of new, distinct groups (or subclades; HC1, HC1, and HC3). To more accurately establish
the taxonomic status and population structure, further genetic, morphological, and ecological studies will be needed. |
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Keywords: | 12S rRNA Asian salamander Hynobius mitochondrial cytochrome b phylogenetic relationship |
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