S100A2 level changes are related to human periodontitis |
| |
Authors: | Sun-Hee Heo Young-Jin Choi Ji-Hyun Lee Jae-Mok Lee Je-Yoel Cho |
| |
Institution: | (1) Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, YO10 5YW, UK;(2) School of Biomedical and Health Sciences, Hodgkin Building, Guy’s Campus, King’s College, London, SE1 1UL, UK |
| |
Abstract: | Periodontitis is an inflammatory disease, which, when severe, can result in tooth loss, that affects the quality of life.
S100A2 was previously identified as a component of gingival crevicular fluid (GCF) via proteome analysis, but it has not been investigated whether S100A2 plays a role in periodontitis. In this study, we analyzed
mRNA expression of S100A2 in gingival tissues from normal and classified periodontal disease patients and compared it to that
of S100A8 and S100A9. Quantitative real time-PCR revealed that the mRNA expression levels of S100A2, S100A8, and S100A9 were
significantly upregulated in gingival tissues with gingivitis, moderate periodontitis, and severe periodontitis compared to
normal tissues. In addition, S100A2 proteins in GCF and the conditioned media of lipopolysaccharide (LPS)-treated Jurkat cells
were confirmed by ELISA. S100A2 protein levels were significantly higher in GCF in gingivitis and moderate periodontitis groups
than in normal groups. S100A2 mRNA expression and protein secretion were also increased by LPS stimulation. Based on the up-regulation
of S100A2 in LPS-stimulated immune cells, gingival tissues and GCF from periodontal disease groups, we conclude that S100A2
is a functional component in the immune response during periodontitis and may serve as a potential biomarker for periodontitis. |
| |
Keywords: | gingival crevicular fluid gingival tissue lipopolysaccharide periodontitis s100a2 |
本文献已被 PubMed SpringerLink 等数据库收录! |
|