不同波段电磁辐射致大鼠睾丸支持细胞的损伤效应

为探讨不同波段电磁辐射对大鼠睾丸支持细胞(Sertoli细胞)损伤效应的异同.将原代培养的Sertoli细胞经场强6×104V/m的电磁脉冲(electromagnetic pulse,EMP)、平均功率密度为100mW/cm2的S-波段高功率微波(S-band high power microwave,S-HPM)和...

The Damaging Effects of Different Bands Electromagnetic Radiation on The Rat Sertoli Cells

To investigate comparatively the damaging effects of EMP (electromagnetic pulse), S-HPM (S-band high power microwave) and X-HPM (X-band high power microwave) on rat Sertoli cells. Primary Sertoli cells were isolated from 3-wk-old Wistar rats, and exposed to EMP, S-HPM and X-HPM respectively. After radiation by the three bands electromagnetic radiation respectively, the number of late apoptotic and necrotic Sertoli cells was increased significantly in three groups examined by flow cytometry; The general metabolic activity of Sertoli cells in EMP group were decrease significantly after irradiation analyzed by MTT test; The total number of impaired Sertoli cells were increased significantly observed by light microscope. Granular degeneration and vacuolar degeneration were seen in the cytoplasm. Ultrastructural changes: mitochondria swelling and vacuolating, endoplasmic reticular various degree dilating (particularly in S-ER) and ribosome-scaling in R-ER and so on. Three bands of electromagnetic radiation could induce different injury effects in rat Sertoli cells. The degree of injury effects varied according to different microwaves. The rank as follows: EMP>X-HPM>S-HPM.