Simultaneous determination of albendazole sulfoxide enantiomers and albendazole sulfone in plasma |
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Authors: | Vera Lucia Lanchote Maria Paula C Marques Osvaldo Massaiti Takayanagui Roberto de Carvalho Fernanda Orsi Paias Pierina Sueli Bonato |
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Institution: | aFaculdade de Ciências Farmacêuticas de Ribeirão Preto, USP, Ribeirão Preto, Brazil;bFaculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, Brazil;cFaculdade de Filosofia, Ciências e Letras de Ribeirão Preto, USP, Ribeirão Preto, Brazil |
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Abstract: | A high-performance liquid chromatographic method has been developed for the simultaneous determination of albendazole sulfoxide (ABZSO) enantiomers and albendazole sulfone (ABZSO2) in human plasma. The resolution of ABZSO enantiomers and ABZSO2 was obtained on a Chiralpak® AD column using hexane–isopropanol–ethanol (81:14.25:4.75, v/v/v) as the mobile phase. The drugs were detected by fluorescence (λexc=280 nm, λem=320 nm). The drugs were extracted from 500 μl plasma with ethyl acetate, and after solvent evaporation, the residues were dissolved in the mobile phase and chromatographed. The method was precise and accurate for the three compounds, as judged by the coefficients of variation and relative errors observed. Linear standard curves were obtained in the concentration range of 5–2500 ng/ml for ABZSO enantiomers and 1–500 ng/ml for ABZSO2. A typical plasma concentration–time profile is presented for one patient under treatment for neurocysticercosis. |
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Keywords: | Albendazole |
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