Photo-induced crosslinking of histones H3 and H1 to DNA in deoxyribonucleoprotein: implication in studying histone-DNA interactions. |
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| Authors: | L Kurochkina G Kolomijtseva |
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| Affiliation: | A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, USSR. |
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| Abstract: | A thymine-modified derivative of histone H3, isolated as a result of heat treatment of covalently crosslinked DNA-protein photoadduct from UV-irradiated chromatin, was obtained. Sequence analysis of one of its tryptic peptides revealed that lysine-14 of the N-terminal tail of the histone H3 molecule covalently binds to thymine residue of DNA. This type of UV-crosslinking is most probably the only type for histone H3 and, possibly, for H1. |
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