A retrovirus-based system to stably silence hepatitis B virus genes by RNA interference |
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| Authors: | Fang Jia Yi-Zheng Zhang Chang-Mei Liu |
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| Institution: | (1) School of Life Sciences, Sichuan University, Chengdu , 610064, China;(2) Molecular Virology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing , 100080 , China |
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| Abstract: | RNA interference (RNAi) might be an efficient antiviral therapy for some obstinate illness. Herein, a retrovirus-based RNAi system was developed to drive expression and delivery of Hepatitis B virus (HBV)-specific short hairpin RNA (shRNA) in HepG2 cells. The levels of HBsAg and HBeAg and that of HBV mRNA were dramatically decreased by this RNAi system in HepG2 cells transfected with Topo-HBV plasmid. Retrovirus-based RNAi thus may be useful for therapy in HBV and other viral infections and provide new clues for prophylactic vaccine development. |
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| Keywords: | Hepatitis B virus HepG2 cells Retrovirus vector RNA interference |
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