The appearance of an enzyme activity catalysing the conversion of norsolorinic acid to averantin in Aspergillus parasiticus cultures |
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Authors: | Anil A Chuturgoon Michael F Dutton |
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Institution: | (1) Department of Biochemistry, University of Natal, Pietermaritzburg, 3200 Natal, South Africa |
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Abstract: | The activity of the enzyme responsible for the conversion of norsolorinic acid to averantin was studied in two strains of Aspergillus parasiticus. Cell-free extracts of the enzyme were purified from different aged mycelia and little activity was found prior to 24 hours after inoculation but this quickly reached a maximum at 48 hours and declined thereafter. Both strains of A. parasiticus, one in aflatoxin producing strain, the other a versicolorin A accumulating mutant, showed this trend. It was concluded that the enzyme responsible for this conversion was a secondary metabolic enzyme and was distinct from alcohol and mannitol dehydrogenases. |
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Keywords: | Aspergillus parasiticus averantin norsolorinic acid secondary metabolic enzyme |
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