Suspension (S)-FISH, a new technique for interphase nuclei. |
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Authors: | Ulf Steinhaeuser Heike Starke Angela Nietzel Joerg Lindenau Peter Ullmann Uwe Claussen Thomas Liehr |
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Affiliation: | Institute of Human Genetics, Jena, Germany. |
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Abstract: | We describe a versatile method for performing fluorescence in situ hybridization (FISH) in suspension instead of on a slide as usually done. This so-called suspension-FISH (S-FISH) opens new possibilities for the analysis of shape and functions of the human interphase nucleus. The procedure is described and the first results using this approach are presented. |
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