医院感染阴沟肠杆菌的耐药性及基因型分析

了解中南大学湘雅三医院重症监护病房(ICU)医院感染阴沟肠杆菌的耐药谱及分子流行病学特征,指导临床合理用药。基因分型采用优化反应体系的随机扩增多态性DNA(RAPD)法,耐药分析选用K-B法。RAPD分型将28株阴沟肠杆菌分为11种株型,耐药谱则将其分为12种不同的耐药型。基因分型和耐药分析显示ICU有多重耐药的阴沟肠杆菌株爆发流行现象,它为控制阴沟肠杆菌的医院内感染、追踪传染原、切断传播途径提供遗传学信息,指导临床医生选用敏感有效的抗生素。

Drug Resistance and Genotyping of Enterobacter cloacae Infected in Intensive Care Units

The type of resistance spectrum and molecular biological characteristics of Enterobacter cloacae infected in intensive care units(ICU) was studied with random amplified polymorphic DNA(RAPD) analysis for gene typing.K-B method was used for the determination of antibiotic resistance pattern.The results showed that 28 strains were typed into 12 kinds.And genotyping and analysis of resistance to antibiotics demonstrated that the outbreak of multidrug resistant E.cloacae strains in ICU.It can not only provide genetic information for controlling,preventing hospital infection and tracing the infection source and the way of dissemination but also direct the clinical doctors to select sensitive antibiotics.