| Affiliation: | 1. Department of Therapeutic Radiology and Oncology, Innsbruck Medical University, , A‐6020 Innsbruck, Austria;2. Division of Histology and Embryology, Innsbruck Medical University, , A‐6020 Innsbruck, Austria;3. Division of Cell Biology, Biocenter, Innsbruck Medical University, , A‐6020 Innsbruck, Austria;4. Institute of Pharmacology, Innsbruck Medical University, , A‐6020 Innsbruck, Austria;5. Department for Trauma Surgery, Innsbruck Medical University, , A‐6020 Innsbruck, Austria |
| Abstract: | ![]()
Electrospun nanofibres are an excellent cell culture substrate, enabling the fast and non‐disruptive harvest and transfer of adherent cells for microscopical and biochemical analyses. Metabolic activity and cellular structures are maintained during the only half a minute‐long harvest and transfer process. We show here that such samples can be optimally processed by means of cryofixation combined either with freeze‐substitution, sample rehydration and cryosection‐immunolabelling or with freeze‐fracture replica‐immunolabelling. Moreover, electrospun fibre substrates are equally suitable for complementary approaches, such as biochemistry, fluorescence microscopy and cytochemistry. |
