Influence of interferon-gamma and extracellular tryptophan on indoleamine 2,3-dioxygenase activity in T24 cells as determined by a non-radiometric assay. |
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| Authors: | E R Werner G Werner-Felmayer D Fuchs A Hausen G Reibnegger H Wachter |
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| Affiliation: | Institute for Medical Chemistry and Biochemistry, University of Innsbruck, Austria. |
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| Abstract: | The indoleamine 2,3-dioxygenase (EC 1.13.11.17) activity in human T24 cells has been investigated in cell extracts by using a non-radioactive assay. It is enhanced in a dose-dependent manner up to 25-fold by interferon-gamma. The maximum reaction velocity is increased rather than the Km, which remains at 4 mumol/l. Induction of activity starts 3 h after stimulation and reaches a plateau at 21-48 h. Decreased stimulation was observed in the presence of high L-tryptophan concentrations. |
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