An improved method for chromosome banding after fluorescence in situ hybridization: Use of a tetramethylrodhamine-conjugated BrdU antibody期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

An improved method for chromosome banding after fluorescence in situ hybridization: Use of a tetramethylrodhamine-conjugated BrdU antibody

Authors:	Albertina De Sario Luigi De Carli Elena Raimondi

Affiliation:	(1) Dipartimento di Genetica e MicrobiologiaA. Buzzati-Traverso, Via Abbiategrasso 207, 27100 Pavia, Italy;(2) Dipartimento di Genetica e Microbiologia A. Buzzati-Traverso, Via Abbiategrasso 207, 27100 Pavia, Italy

Abstract:	A method for high quality chromosome banding after in situ hybridization with biotinylated probes has been developed. Fluoresceine-conjugated avidin is used for probe detection, while chromosome banding is performed with a tetramethylrodhamine-conjugated anti-BrdU antibody. In this way probe localization and chromosome identification can be performed simultaneously simply by changing the incidental light wavelength.Abbreviations BAT BrdU antibody technique - DABCO 1,4 diazobicyclo-(2.2.2)octane - FITC fluorescein isothiocyanate - FPG fluorochrome plus giemsa - PHA phytohemagglutinin - RBA R-banding BrdU acridine - TRITC tetramethylrhodamine isothiocyanate

Keywords:	chromosome banding biotinylated DNA probes BrdU antibodies fluorescence in situ hybridization
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