Quantification of the virus-host interaction in human T lymphotropic virus I infection |
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Authors: | Becca Asquith Angelina J Mosley Adrian Heaps Yuetsu Tanaka Graham P Taylor Angela R McLean Charles RM Bangham |
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Institution: | 1. Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Hills Road, Box 157, CB2 2QQ, Cambridge, UK
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Abstract: | Background Cellular infection with human immunodeficiency virus (HIV) both in vitro and in vivo requires a member of the chemokine receptor family to act as a co-receptor for viral entry. However, it is presently unclear to what extent the interaction of HIV proteins with chemokine receptors generates intracellular signals that are important for productive infection. Results In this study we have used a recently described family of chemokine inhibitors, termed BSCIs, which specifically block chemokine-induced chemotaxis without affecting chemokine ligands binding to their receptors. The BSCI termed Peptide 3 strongly inhibited CCR5 mediated HIV infection of THP-1 cells (83 ± 7% inhibition assayed by immunofluoresence staining), but had no effect on gp120 binding to CCR5. Peptide 3 did not affect CXCR4-dependent infection of Jurkat T cells. Conclusion These observations suggest that, in some cases, intracellular signals generated by the chemokine coreceptor may be required for a productive HIV infection. |
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