Changes in microRNA expression profiles in HIV-1-transfected human cells |
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Authors: | Man Lung Yeung Yamina Bennasser Timothy G Myers Guojian Jiang Monsef Benkirane Kuan-Teh Jeang |
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Institution: | 1. Department of Molecular Biology, University of Bergen, N-5020, Bergen, Norway 2. Department of Molecular Biology, University of Aarhus, DK-8000, Aarhus C, Denmark 3. EMBL, Heidelberg, Germany
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Abstract: | Background The HIV-1 Rev regulatory protein binds as an oligomeric complex to viral RNA mediating nuclear export of incompletely spliced and non-spliced viral mRNAs encoding the viral structural proteins. However, the biological significance of the obligatory complex formation of Rev upon the viral RNA is unclear. Results The activity of various fusion proteins based on the negative oligomerization-defect Rev mutant M4 was tested using Rev dependent reporter constructs. An artificial M4 mutant dimer and an M4 mutant containing an extra basic domain from the HTLV-I Rex protein exhibited nearly full activity when compared to wild type Rev. Conclusion Rev dimerization appears to be required to expose free basic domains whilst the Rev oligomeric complex remains bound to viral RNA via other basic domains. |
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