Isolating Influenza RNA from Clinical Samples Using Microfluidic Oil-Water Interfaces |
| |
| Authors: | Francis R Cui Jingjing Wang Steven M Opal Anubhav Tripathi |
| |
| Institution: | 1. Center for Biomedical Engineering, School of Engineering, Brown University, Providence, Rhode Island, United States of America;2. Warren Alpert Medical School, Brown University, Providence, Rhode Island, United States of America;3. Memorial Hospital of Rhode Island, Pawtucket, Rhode Island, United States of America;Naval Research Laboratory, UNITED STATES |
| |
| Abstract: | The effective and robust separation of biomolecules of interest from patient samples is an essential step in diagnostic applications. We present a platform for the fast extraction of nucleic acids from clinical specimens utilizing paramagnetic PMPs, an oil-water interface, a small permanent magnet and a microfluidic channel to separate and purify captured nucleic acids from lysate in less than one minute, circumventing the need for multiple washing steps and greatly simplifying and expediting the purification procedure. Our device was able to isolate influenza RNA from clinical nasopharyngeal swab samples with high efficiency when compared to the Ambion® MagMAXTM Viral RNA Isolation Kit, sufficiently separating nucleic acid analytes from PCR-inhibiting contaminants within the lysate while also critically maintaining high integrity of the viral genome. We find that this design has great potential for rapid, efficient and sensitive nucleic acid separation from patient sample. |
| |
| Keywords: | |
|
|
