Efficient plant regeneration of Mesembryanthemum crystallinum via somatic embryogenesis |
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| Authors: | J C Cushman T Wulan N Kuscuoglu M D Spatz |
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| Institution: | (1) Department of Biochemistry and Molecular Biology, 147 Noble Research Center, Oklahoma State University, Stillwater, Oklahoma, USA Fax: +1–405–744–7799 e-mail: jcushman@biochem.okstate.edu, US;(2) Department of Biochemistry, 540 Biosciences West, University of Arizona, Tucson, AZ 85721 USA, US |
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| Abstract: | An efficient plant regeneration procedure has been established from hypocotyl explants of the common ice plant, Mesembryanthemum crystallinum L, a halophytic leaf succulent that exhibits a stress-induced switch from C3 photosynthesis to crassulacean acid metabolism
(CAM). Somatic embryos were initiated and developed up to globular and heart stages in Murashige and Skoog (MS) media supplemented
with 3% sucrose, 0.6% bacto-agar, 80 mM NaCl, 5 μM 2,4-D and 1 μM kinetin. High frequency regeneration occurred when somatic
embryos were germinated on media that lacked 2,4-D. High cytokinin treatment suppressed normal growth of embryos and favored
abnormal embryo proliferation. Without growth regulators, regenerated plants rooted on MS medium with 100% efficiency. Mature,
regenerated plants were fertile and morphologically identical to seed-derived plants.
Received: 29 April 1999 / Revision received: 2 July 1999 · Accepted: 12 July 1999 |
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| Keywords: | Mesembryanthemum crystallinum Aizoaceae Somatic embryogenesis Halophyte Facultative CAM plant |
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