CD44在隐球菌性脑膜炎发病机制中的作用研究

目的测定隐球菌性脑膜炎小鼠脑组织中CD44表达,探讨CD44在隐球菌性脑膜炎发病机制中的作用。方法隐球菌性脑膜炎免疫抑制小鼠为实验组,未接种隐球菌的免疫抑制小鼠为对照组,应用免疫组化法检测实验组6h、12h、24h、48h、72h、4d、7d小鼠脑组织CD44表达与对照组的变化。结果对照组小鼠脑组织CD44均匀分布在脑细胞膜上。实验组隐球菌作用小鼠48h、72h后,CD44在小鼠脑组织脑膜侧分布增加,而在脑实质侧CD44的分布明显减少。病灶周围的脑组织CD44分布也一侧增加,另一侧分布减少。结论隐球菌侵入小鼠脑组织后,隐球菌诱导CD44向脑组织的一侧移行,向脑膜方向聚集。病灶周围脑组织CD44分布也不均匀。说明隐球菌性脑膜炎的发生与CD44有密切的关系。

CD44 expression in murine model of cryptococcal meningitis

Objective To determine the CD44 expression in the brain of mice with cryptococcal meningitis and to explore its role in pathogenesis of cryptococcal meningitis.Methods CD44 expression in immunocompromised mice with or without cryptococcal meningitis were determined at 6 h,12 h,24 h,48 h,72 h,4 d,7 d by immunohistochemistry.Results CD44 distributed uniformly in the brain cell membrane in the control group.The expression increased in the meningeal side,while decreased significantly in the parenchyma side after 48 h and 72 h in the experimental group.This unbalanced distribution of CD44 expression was also observed around lesions.Conclusions Cryptococcus neoformans could induce CD44 migration in the brain.The incidence of cryptococcal meningitis has effects on CD44 molecules.