Stimulation of endothelial cell migration in culture by ladsin,a laminin-5-like cell adhesion protein |
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Authors: | Yamato Kikkawa Kotaro Akaogi Hiroto Mizushima Naoki Yamanaka Makoto Umeda Kaoru Miyazaki |
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Institution: | (1) Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244 Yokohama, Japan;(2) Present address: Department of Physiology, Tokushima University School of Dentistry, Kuramoto-cho, 770 Tokushima, Japan |
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Abstract: | Summary Ladsin is a laminin-like cell-adhesive scatter factor with potent cell motility-stimulating ability and was purified from
serum-free conditioned medium of a malignant human gastric adenocarcinoma cell line STKM-1. To test its possible role in tumor
angiogenesis, we investigated its effect on primary culture of endothelial cells (human umbilical vein endothelial cells)
and endothelial cell line ECV304 in this study. Cell adhesion and motility effects of ladsin were observed in both types of
endothelial cells. In cell-attachment assay, ladsin interacted with integrin α3β1 that was expressed on the endothelial cell
surface. In Boyden chambers, ladsin stimulated both directed and random migration of ECV304 cells. Ladsin induced repair of
artificial wounds generated in ECV304 cell monolayers by stimulating cell migration. Ladsin did not affect the growth rate
of ECV304 cells at a low cell density but significantly increased the saturation cell density. These results suggest that
ladsin may be involved in the adhesion and migration of endothelial cells under some physiological and pathological conditions. |
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Keywords: | endothelial cell cell migration cell adhesion laminin tumor angiogenesis |
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