Characteristics of protein partitioning in an aqueous micellar-gel system |
| |
| Authors: | van den Broeke L J P van Roosmalen D Dohmen-Speelmans M P J Dietz C H J T van der Wielen L A M Keurentjes J T F |
| |
| Institution: | Department of Chemical Engineering and Chemistry, Process Development Group, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands. l.p.j.van.den.broeke@tue.nl |
| |
| Abstract: | Partitioning of proteins has been studied experimentally in a system combining a gel-bead phase and a nonionic micellar phase. The micellar phase consists of cylindrically shaped micelles, which are completely excluded from the gel-bead phase. Partitioning of single-component protein solutions (myoglobin, ovalbumin, and BSA) is determined by excluded-volume interactions in the micellar phase, and as a result the proteins prefer the gel-bead phase to the micellar phase. The protein concentration inside the gel beads increases with an increase in volume fraction of the micelles and increases with an increase in the size of the proteins. The protein partition coefficients obtained for a binary mixture of myoglobin and bovine serum albumin (BSA) show the same protein concentration dependence as the single-component protein partition coefficients. |
| |
| Keywords: | nonionic surfactant aqueous micellar‐gel systems size exclusion excluded‐volume interactions protein purification sephacryl large pore gel |
| 本文献已被 PubMed 等数据库收录！ |
|
