Monoclonal antibodies to surface and cytoskeletal components of the spermatozoid ofPteridium aquilinum |
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Authors: | J Marc B E S Gunning A R Hardham J L Perkin S M Wick |
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Institution: | (1) Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, GPO Box 4, 2601 Canberra, ACT, Australia;(2) Present address: Department of Botany, University of Georgia, 30602 Athens, GA, USA;(3) Present address: Department of Botany, University of Minnesota, 55108 St Paul, MN, USA |
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Abstract: | Summary Detergent extracted spermatozoids of the fernPteridium aquilinum were used as mixed antigen preparations for raising monoclonal antibodies in order to obtain reagents for detecting as yet uncharacterized components of the plant cytoskeleton. Selected antibodies were studied by immunofluorescence microscopy of developing spermatids and mature spermatozoids. Some reacted directly with fixed cells, others required permeabilization treatments with cold methanol or Triton X-100. AntibodiesPas2D9 andPas6D7 bind to glycoprotein antigenic determinants that are exposed on the surface of the plasma membrane. Several antibodies interact with cytoskeletal components.Pas1D3,Pas5D8 andPas5F4 bind to the cytoskeleton of permeabilized cells including the flagella. Three react specifically with the flagellar band or associated components:Pas2G6 reacts with the whole flagellar band but shows a prominent binding to basal bodies,Pas5E2 binds exclusively to basal bodies, andPas5E7 detects mitochondria associated with the flagellar band. Cross-reactions to wheat root tip cells at different stages of the cell cycle are described inMarc andGunning (1988).Abbreviations MLS
multilayered structure
- MT
microtubule
- MAb
monoclonal antibody
- MAP
microtubule associated protein |
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Keywords: | Basal body Cytoskeleton Microtubule Immunoflu orescence Monoclonal antibodies Pteridium Spermatozoid |
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