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Cloning of the cDNA encoding neural adhesion molecule F3 from bovine brain |
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| Authors: | Kazutada Watanabe Kuniko Shimazaki Hiroko Hosoya Fumihiko Fukamauchi Tadaomi Takenawa |
| Institution: | a Department of Experimental Biology, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173, Japan b Department of Physiology, Jichi Medical School, Minamikawachi-machi, Tochigiken 329-04, Japan. Tel. (81-285) 44-2111 c Department of Molecular Medical Science, Medical Research Institute, Tokyo Medical and Dental University, Kandasurugadai, Chiyoda-ku, Tokyo 101, Japan. Tel. (81-3) 5280-8059 d Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Shiroganedai, Minato-ku, Tokyo 108, Japan. Tel. (81-3) 5449-5510 |
| Abstract: | We cloned a bovine cDNA encoding the neural adhesion molecule F3 and analyzed its nucleotide sequence. The coding region consisted of 3054 bp encoding 1018 amino acid (aa) residues. The Mr calculated from the deduced as sequence was 113 383. Bovine F3 had 93, 94 and 77% as identity with the mouse, human and chicken homologs, respectively. Bovine F3, similar to those of chicken and human, was devoid of two as residues (Ile-Thr) in the sixth immunoglobulin type C2-like domain, as compared with the mouse homolog. Parts of bovine F3 protein were overproduced in Escherichia coli. The antibodies raised against the recombinant proteins in rabbits reacted specifically with F3. F3 protein was detected in cerebellum, cerebrum and spinal cord in Western blot analysis. |
| Keywords: | F11 contactin Ig superfamily GPI anchor cell adhesion brain function |
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