Rat and human embryo and post-natal sera contain a potent endogenous competitor of estrogen-rat alpha-fetoprotein interactions |
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| Authors: | C. Benassayag G. Vallette J. Delorme L. Savu E.A. Nunez M.F. Jayle |
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| Affiliation: | Laboratoire associé n° 87 du C.N.R.S. U.E.R. Biomédicale des Saints-Pères 45, rue des Sts-Pères 75270 PARIS Cedex 06, France |
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| Abstract: | ![]()
A highly active inhibitor of the binding of estrone and estradiol-17β to rat alpha-fetoprotein is demonstrated for the first time in embryo, immature and adult rat sera as well as in fetal and adult human sera. The competitive character and the narrow specificity of this inhibition effect is shown. The major compound responsible for this activity is isolated by successive column Sephadex LH20 and thin layer chromatography : it is characterized as a nonpolar, nonphenolic, dialysable and thermostable substance, unreactive towards anti-estrone and anti-estradiol-17β anti-bodies. The possible biological role of an endogenous non-estrogen ligand of rodent fetoproteins is discussed. |
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| Keywords: | FSH follicle-stimulating hormone LH Luteinizing hormone HCG human chorionic gonadotrophin 20αHSD 20α-hydroxy-steroid-dehydrogenase 17β-HSD 17β-hydroxysteroid-dehydrogenase Pregnenolone 3β-hydroxy-5-pregnen-20-one Progesterone 4-pregnene-3,20-dione 17-hydroxyprogesterone 17-hydroxy-4-pregnene-3,20-dione 20α-dihydroprogesterone 20α-hydroxy-4-pregnen-3-one Androstenedione 4-androstene-3,17-dione Testosterone 17β-hydroxy-4-androsten-3-one Estrone 3-hydroxy-l,3,5(10)-estratrien-17- one Estradiol 1,3,5(10)-estratriene-3,17β-diol Dihydrotestosterone 17β-hydroxy-5α-androstan-3-one etinylestradiol 17-ethinyl-1,3,5(10)-estratriene-3,17β-diol Tris buffer tris (hydroxy methyl) amino methane (Sigma) NAD nicotinamide-adenine-dinucleotide NADP nicotinamide-adeninedinucleotide phosphate Silica-gel Silicar-TCC 76F (Mallinckrodt) T. L. C. Thin-layer chromatography using 20 × 20 cm glass plates coated with 0.25 mm support medium glucose-6-phosphate D-glucose-6-phosphate dipotassium salt, hydrate, molecular weight (with 3 H20/M) = 390.6 (Sigma) Glucose-6-phosphate dehydrogenase type V, crystalline, from Baker's yeast activity |
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